BUFFEVANT COLLAGEN PDF

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Differential scanning calorimetry DSC has been applied to the study of connective tissue to evaluate the denaturation process of collagen. We have applied this technique to the study of the ageing of rat skin. We have tried to correlate the variations of the parameters measured by DSC and the modifications of collagen crosslinks with ageing. The thermograms obtained are composed of one main peak located between two shoulders. On the other hand, the assay of reducible collagen crosslinks in rat skin, over the same age range, shows a decrease of heat-labile aldimine crosslink essentially hydroxylysinonorleucine.

These results and the study of thermograms obtained with altered rat skin animals treated with beta-aminopropionitrile or skin reduced with NaBH4 allow us to conclude that heat-labile and heat-stable crosslinks account for a collagen thermal stabilization which can explain the delay of denaturation characterized by the third peak of DSC thermograms.

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Abstract Differential scanning calorimetry DSC has been applied to the study of connective tissue to evaluate the denaturation process of collagen.

Similar articles Influence of collagen denaturation on the chemorheological properties of skin, assessed by differential scanning calorimetry and hydrothermal isometric tension measurement. Biochim Biophys Acta. PMID: Biosynthesis of collagen crosslinks: in vivo labelling of neonatal skin, tendon, and bone in rats. Reiser KM, et al. Connect Tissue Res. Tsereteli GI, et al. PMID: Russian. Connective tissues--possible implications of the temporal changes for the aging process.

Viidik A. Mech Ageing Dev. PMID: Review. Molecular aging and senescence. Fels IG. No abstract available. Show more similar articles See all similar articles. Cited by 6 articles Structural features of eyelid connective tissue in patients with primary open-angle glaucoma. Svetikova LA, et al. Int Ophthalmol. Epub Oct Takaoka A, et al. Invest Ophthalmol Vis Sci. Increased C-telopeptide cross-linking of tendon type I collagen in fibromodulin-deficient mice.

Kalamajski S, et al. J Biol Chem. Epub May The effect of cross-link distributions in axially-ordered, cross-linked networks. Bennett CB, et al. J Phys Condens Matter. Epub Jun Thermal transitions of fibrillar collagen unveiled by second-harmonic generation microscopy of corneal stroma. Matteini P, et al. Biophys J. Show more "Cited by" articles See all "Cited by" articles. Publication types Research Support, Non-U. Gov't Actions. Animals Actions. Calorimetry, Differential Scanning Actions.

Male Actions. Protein Denaturation Actions. Rats Actions. Rats, Inbred Strains Actions. Thermodynamics Actions. Substances Dipeptides Actions. Desmosine Actions. Histidine Actions. Collagen Actions. Full-text links [x] Elsevier Science. Copy Download.

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